本书概述了设计PCR引物以成功扩增DNA的方法。各章重点介绍定量PCR的引物设计策略、硅片PCR引物设计以及使用软件进行引物设计。本书采用非常成功的《分子生物学方法》系列格式编写，各章包括各自主题的介绍、必要的材料和试剂清单、逐步的、易于重复的实验方案，以及排除故障和避免已知陷阱的提示。

《PCR引物设计，第二版》具有权威性和易懂性，旨在帮助分子生物学学生、研究人员、教授和PCR爱好者。

This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.